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KMID : 1134820120410070901
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Journal of the Korean Society of Food Science and Nutrition
2012 Volume.41 No. 7 p.901 ~ p.906
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Ishige sinicola Extracts Induce Apoptosis via Activation of a Caspase Cascade in Human HeLa Cells
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Cho Byoung-Ok
Ryu Hyung-Won
So Yang-kang
Jin Chang-Hyun
Byun Myung-Woo
Kim Wang-Geun
Jeong Il-Yun
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Abstract
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The purpose of this study was to elucidate the anti-proliferative effect and the mechanisms underlying apoptosis induced by a methanol extracts from Ishige sinicola (ISE) in HeLa cells. ISE treatment for 24 hr significantly inhibited cell viability in a dose-dependent manner. Apoptosis was detected by Hoechst 33258 staining and an annexin V/PI assay after 24 hr treatment. Moreover, ISE treatment triggered the cleavage of caspase-8, -9, -3, and poly(ADP-ribose) polymerase (PARP) in dose-dependent and time-dependent manners. In addition, z-VAD-fmk, a general caspase inhibitor, blocked ISE-induced cell death. Taken together, these results suggest that ISE-induced apoptosis is mediated by the activation of a caspase cascade in HeLa cells.
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KEYWORD
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Ishige sinicola, apoptosis, caspase, poly(ADP-ribose) polymerase (PARP)
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